Fig. 3

Fig. 3

Growth and cardiometabolic phenotype are significantly impacted by early life grb10a knockdown. (A) Mean total body length ± SEM of SC and KD zebrafish from 24 to 120 hpf (multiple comparison 2-way ANOVA, * p < 0.05, *** p = 0.0003). (B) Mean total body length ± SEM and individual data points of 96 hpf zebrafish embryos (n = 25). Grb10a KD phenotype was reversed in grb10a overexpression zebrafish. Co-injection resulted in phenotype rescue. One-way ANOVA revealed KD zebrafish were significantly longer, while grb10a overexpression zebrafish were significantly smaller than SC (*** p = 0.0001). Rescue zebrafish were of similar length to SC (p = 0.38). (C) Mean body length measurements of the KD relative to SC ± SEM from 48 to 120hpf. (D) Mean heart rate ± SEM in beats per minute of SC and KD zebrafish (multiple comparison 2-way ANOVA, **** p < 0.0001). (E) Mean yolk area ± SEM of SC and KD embryos over the embryonic life stage (multiple comparisons 2-way ANOVA, *** p = 0.0004. (F) Glucose Uptake-Glo.™ Assay of 96 hpf KD and SC zebrafish, where higher luminescence indicates a greater accumulation of intracellular 2D6P. Luminescence was approximately 30% greater in KD zebrafish compared to SC (unpaired t-test, *** p = 0.0002)