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Fig. 3 RPE expressed rlbp1b knockout larvae have an OKR visual behavior defect specifically in dim light. (A) Schematic showing raising conditions, followed by the type of OKR assay. (B) Standard light OKR assay showing no difference between rlbp1b−/− larvae with wildtype siblings. Four biological replicates n ≥ 17 larvae per group. (C) Dim light OKR assay comparing rlbp1b−/− larvae with WT siblings. Four biological replicates n ≥ 16 larvae per group. p < 0.0001 unpaired two tailed t-test. (D) No difference is seen in dim light OKR assays between rlbp1a−/− larvae with WT siblings. Three biological replicates n ≥ 10 larvae per group. (E) Dim light OKR assay showing a significant difference between rlbp1a−/−;rlbp1b−/− larvae and rlbp1b−/− larvae. Four biological replicates n ≥ 17 larvae per group. p = 0.0018 unpaired two tailed t-test. (F) Schematic showing raising conditions including 24 h bright light treatment starting on 99 hpf, followed by type of OKR assay. (G) 24 h bright light treatment followed by standard light OKR assay showing no difference between rlbp1a−/−;rlbp1b−/− larvae and rlbp1b−/− larvae. Three biological replicates n ≥ 11 larvae per group. (H) 24 h bright light treatment followed by dim light OKR assay showing a significant difference between WT siblings with rlbp1b−/− larvae. Three biological replicates n ≥ 10 larvae per group. p < 0.0001 unpaired two tailed t-test. All OKRs were carried out with a 0.02 CPD drum on < 131 hpf larvae. All graphs show mean ± standard deviation.