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Sweeney et al., 2025 - Creb3l3 deficiency promotes intestinal lipid accumulation and alters ApoB-containing lipoprotein kinetics

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Fig. 2

Generation of creb3l3a and creb3l3b zebrafish mutants. A: Representative images of whole-mount in situ hybridization (ISH) with sense and antisense probes targeting zebrafish creb3l3a (ENSDARG00000056226.7) and creb3l3b (ENSDARG00000090903.3) at 6 days post fertilization (dpf). Both creb3l3a and creb3l3b mRNA is expressed in the liver and the intestine at 6 dpf (white outlines). No signal is observed with sense probes. Images are representative of 3 independent clutches: 6–12 animals per clutch. B: Overview of gene loci and strategy used for CRISPR/Cas9 targeting of creb3l3a and creb3l3b. The fifth exon was targeted by CRISPR guide RNA in creb3l3a, and the sixth exon was targeted in creb3l3b. C: Sanger sequencing confirms 2-bp deletions in both creb3l3a and creb3l3b (nucleotides depicted in gray show the affected codon causing a frameshift; dotted box around sequence trace confirms codon change by loss of 2 base pairs). Creb3l3, cAMP-responsive element-binding protein 3–like 3.

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Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Lipid Res.