Figure 2

Figure 2

Lurasidone induces dose-dependent neuronal apoptosis in zebrafish embryos. (A) Representative images of 120 hpf zebrafish embryos under differential interference contrast (DIC) and fluorescence microscopy following acridine orange (AO) staining. Embryos were exposed to different concentrations of Lurasidone (0.4, 4, and 8 mg/L) or vehicle control. Apoptosis was visualized by acridine orange staining in live embryos, where apoptotic cells are indicated by green fluorescence, with merged images displaying the anatomical localization of apoptotic signals. (B) Quantitative analysis of relative apoptosis levels (%) across treatment groups. Data are presented as box plots showing median, quartiles, and individual data points (n=12 per group). Statistical significance is indicated by asterisks (***: P < 0.001) compared to control group.