|
Fig. 3 (A) The recovery of BuChE inhibitors (donepezil, rivastigmine and 8e) inhibition after dilution with time monitoring. (B) The cell viability (%) of 8e on HT22 cells, PC12 cells and SH-SY5Y cells. (C) Neuroprotective effects of 8e and donepezil on Glu-induced HT22 cell injury. (D) Neuroprotective effects of 8e and donepezil on Aβ25–35-induced HT22 cell injury. (E) Neuroprotective effects of 8e on Aβ25–35-induced PC12 cell injury. (F) The chemical stability of 8e in artificial gastrointestinal fluid. (G) The metabolic stability of 8e in human/rat liver microsomes. (H) The CYP inhibition effects of 8e. (I) In vivo PET-CT images of [11C]8e in mice brain after intravenous administration and time–activity curve. Values are presented as mean ± SD from three independent experiments. ##P < 0.01 vs. control group; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. model group.