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Fig. 8 Effect of N3 in PTZ-induced epilepsy-like condition in zebrafish larvae by reducing the calcification and β-amyloid plaques. The graph represents (A) Alizarin red staining in zebrafish larvae (scale 50 μm), (B) Thioflavin S staining in zebrafish larvae (scale 100 μm), (C) Percentage of alizarin red stained region in larvae using Image J software and (D) Percentage of β-amyloid plaques in individual. Experimental groups (n = 15/group) included zebrafish larvae treated with N3 at 50 µM, N3 at 100 µM, PTZ (6 mM), PC - Valproic acid at 100 µM, and control group. Each assay was conducted in triplicate with three independent experiments. Data are expressed as mean ± standard deviation (SD). Statistical significance was determined using one-way ANOVA followed by Dunnett’s multiple comparison test. Significant differences between tested samples and the negative control are indicated by ****p < 0.0001