Fig. 6

Fig. 6 nr6a1(a + b) zebrafish morphants have kidney and vertebrae defects.

At 48 hpf, control embryos demonstrate bilateral expression of nphs1 (nephrin) and nphs2 (podocin) (a, f), markers of kidneys. Knockdown of nr6a1(a + b) resulted in a range of abnormal expression patterns including mildly reduced expression (b, g), moderately reduced expression (c, h), unilateral or midline expression (d, i) or absent expression (e, j) of nphs1 and nphs2. Somites (the early precursors of vertebrae) have a sharp, chevron shape with an approximately 90-degree angle in control zebrafish at 24 hpf (k). Knockdown of nr6a1a and nr6a1b resulted in a range of abnormal expression patterns including chevron shapes with obtuse angles (l), as well as mildly rounded (m) or flattened (n) somites. The sclerotome marker pax9 is expressed in a continuous angular pattern in the ventromedial portion of somites along the length of 24 hpf control embryos (o). Double morphant embryos exhibit a spectrum of abnormal patterns of pax9 expression including reduced levels of expression (p), patchy expression extending beyond the caudal tip of the yolk (q) and patchy expression ending near the caudal end of the yolk sac (r). nr6a1(a + b) morphants have reduced number of somites (s), statistical significance is calculated using two tailed t-test. Std-MO n = 20 and nr6a1-TB-MO(a + b) n = 50. Data is represented as difference between means of nr6a1-TB(a + b)–Std-MO ± SEM (−4.490 ± 0.5629). P value is <0.0001. 0.75 ng of each paralogue is used for knock-down. Numbers of each representative pattern are given in each panel. Scale bar = 100 µm. Source data file has been provided for (s).