Fig. 4

Fig. 4

Hindbrain Ca²⁺ imaging upon TMZ exposure. (A) Representative z-projection images of the hindbrain calcium imaging performed in Tg(neurod1:GCaMP6f) larvae rostral-dorsally settled before the treatment (PRE-TMZ) and after the treatment (POST-TMZ). On the right side, the look up table color range of Ca²⁺ fluorescence is shown. The dotted line indicates the analyzed ROI, while the white arrows indicate the Mauthner cells localization. (B) A representative graph from a single zebrafish larva shows the ∆F/F0 Ca²⁺ fluorescent signal in hindbrain for each frame before and after TMZ treatment. (C) The graph shows full distribution of data, with median and quartiles of ∆F/F0 fluorescent signal in in the hindbrain. (D) The graph shows full distribution of data, with median and quartiles of frequency fluorescent signal in in the hindbrain n = 10 analyzed larvae. Statistical analysis was performed using the paired Wilcoxon test. *p ≤ 0.05, ns: not significant (p of ∆F/F0 = 0.13; p of frequency = 0.03).