Figure 3.

Figure 3. Sephin1 reduces extranuclear TDP-43 localization in primary motor neurons from WT and SOD1^G93A rats.

(A) Representative pictures of TDP-43 localization in control primary SOD1^G93A rat motor neurons, in primary SOD1^G93A rat motor neurons intoxicated by glutamate and in primary SOD1^G93A rat motor neurons intoxicated by glutamate in presence of Sephin1 100 nM. White arrows: TDP-43 localization in cytoplasm of MAP-2 labeled motor neurons, white star: TDP-43 localization in nucleus of MAP-2 labeled motor neurons. (B) Extranuclear TDP-43 level in primary WT rat motor neurons intoxicated by glutamate and treated with DMSO, Sephin1, or riluzole 5 μM for 24 h. (C) Extranuclear TDP-43 level in primary SOD1^G93A rat motor neurons intoxicated by glutamate and treated with DMSO, Sephin1, or riluzole 5 μM for 24 h *P < 0.05, **P < 0.01, ***P < 0.001 versus 0 nM Sephin1 Kruskal-Wallis test followed by Dunn’s multiple comparison test; mean ± SEM n = 4–6 replicates per condition.