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Fig. 5 Multiple reference chemicals evoke inappropriate hyperactivity during light and interstimulus and inter-endpoint intervals (a) Hierarchical clustering of behavioral profiles across the 26-endpoint assay (x-axis) is presented for seventeen reference chemicals that generates increased locomotor activity during the light phase (VMR1), Inter Stimulus Intervals (ISI), Inter Endpoint Intervals (IEI) or Inter Bouts Intervals (IBI). Heatmap colors, based on effect sizes (SSMD), strictly standardized median difference), indicate deviations from the median control phenotype. Red and blue hues represent increased and reduced motor activity, respectively (n = 32–36 larvae). (b, c) MDS representation of pairwise distances between behavioral profiles based on data shown in a. Fill color indicates deviation from the median control phenotype in the (b) VMR1 and (c) IEI2 endpoint where red and blue reflect increase and decrease motor activity, respectively. Light phase fragment of the VAMR test, showing VMR1 endpoint for (d) GSK0660 (25.1 µM, red) and (j) MK-801 (44.8 µM, red). Acoustic fragment of the VAMR test, showing ASR1–3, ASH bouts 1–5, and interstimulus intervals (ISI1–3), as well as quiescent endpoints (IEI1–3, IBI), for (f) GSK0660 (25.1 µM, red) and (l) MK-801 (44.8 µM, red). Raincloud plots representing motor activity responses for zebrafish exposed to 0.4 % DMSO (vehicle control, grey), GSK0660 (25.1 µM, red), and MK-801 (44.8 µM, red) across the endpoints (e, k) VMR1, (g, m) ISI2, (h, n) IEI2, and (i, o) IBI. Motor activity (y-axis) is shown for each endpoint, with the horizontal dotted line indicating the median control habituation score. The adjusted p-value (from a two-sample bootstrapping test) is displayed above each raincloud plot.