Figure 3

Figure 3

Prophylactic treatment with TnP. Retinopathy was induced in 3 dpf larvae through exposure to E2 0.5x medium at 28 °C with 0.5 mM cobalt chloride (CoCl₂) for 72 h, and one group was chosen to be prophylactically treated with 100 µM TnP for same period (A). Independent group of 3 dpf larvae was exposed to intense light at 8000 lux for 24 h (LIRD), and one group was chosen to be prophylactically treated with 100 µM TnP for same period (B). Immediately after stimulation, all groups of larvae were evaluated for their visual–motor response (C,D) and distance traveled (E) and were subsequently killed and processed for histological analysis and H&E staining (F). * p < 0.05 compared to control group. Bars represent mean ± SEM. Dots represent individual data points. RGL: retinal ganglion layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer plexiform layer; OSL: outer segment layer; RPE: retinal pigment epithelium.