Brain histology after 6 weeks of consumption of banaba (BNB, 0.1% w/w), policosanol (PCO, 0.1% w/w), and mixture of the banaba and policosanol (BNB+PCO, each 0.1% w/w) supplemented with high cholesterol (HC, 4% w/w) and high galactose (HG, 30% w/w) diet. (A,B) Hematoxylin and eosin (H&E) staining at 40× and 100× magnification, respectively. (C) Dihydroethidium (DHE) staining. (D) Acridine orange (AO) staining and (E) merge images of DHE and AO staining. (F,G) Quantification of DHE and AO fluorescent intensities. The ** and *** underscore the significant variance at p < 0.01 and p < 0.001, vs. HC+HG group. The † and †† highlight significant differences at p < 0.05 and p < 0.01, vs. BNB+PCO.
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