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Fig. 8 Novel tank test (NTT) results. Ten zebrafish in each group participated in the test. (A) The movement trajectory of zebrafish and a diagram of the test device (Created using BioRender.com). (B) Swimming time in the top region. (C) Driving distance in the top region. (D) Latency (time required for first access to the top). (E) The number of shuttles between the top and bottom region. (F) Preference of zebrafish for top region. (G) Comparison of the distance traveled by each zebrafish in the top and bottom regions. (H) Comparison of time spent in the top and bottom regions for each zebrafish. # denotes a significant difference between the control group and the other groups, while * denotes a significant difference between the T2DM and T2DM+ST groups. Significance thresholds are marked as follows: ns p > 0.05, p < 0.05 (* or #), p < 0.01 (** or ##), p < 0.001 (*** or ###), and p < 0.0001 (****).