Inhibition of biofilm formation by combining phiLCL12 and 1/2 IMP at various MOIs. P. aeruginosa LCL12 at 2 × 107 CFU was co-cultured with phiLCL12 at five different multiplicities of infection (MOIs) for 12 h. (A) Qualitative analysis shows effective suppression of biofilm formation on coverslips. (B) The quantitative analysis supports the qualitative results, demonstrating the extent of biofilm inhibition across different MOIs. All results are from three independent experimental replicates. On the left panel, *** is the control group versus the treated groups assessed by one-way ANOVA, “***” indicates p < 0.001. On the right panel, ### is the phage-only group versus the PAS group assessed by two-way ANOVA. “###” indicates p < 0.001.
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