Fig. 6.

Fig. 6.

Elevated MGAT4B levels correlate with poor patient survival and are crucial for initiating primary tumors. (A) Scatter plot depicting expression of MGAT4B in Skin cutaneous melanoma (SKCM) patients with respect to normal individuals, data procured from TCGA. (B) 60 d old Mazerati zebrafish displaying melanoblast-derived tumor developed by combinations of vectors expressing the oncogene BRAF^V600E, targeting tp53 and ptena/b initiate melanoma Left—mitfa-BRAF^V600E, mitfa-p53^−/−, mitfa-ptena/b^−/− and mitfa:EV, Right—mitfa:mgat4b^−/−. (C) Melanoma-free survival curves of ASWT zebrafish injected with the indicated combinations of vectors expressing mitfa-BRAF^V600E, mitfa-p53^−/−, mitfa-ptena/b^−/− and mitfa:EV or mitfa:mgat4b^−/−. (D) Schematic illustration of the experimental approach adapted to isolate and enrich(percol based gradient) melanophores from one month old wildtype and Mazerati fishes. (E) PCA plot depicting relationship between the three samples sequenced. (F) Euler plot depicting the number of differentially expressed genes in melanocytes/melanoma cells from Mazerati fishes (control and mgat4b mutant (M4b mut) melanoma) with respect to wildtype melanophores. (G) Dot plot depicting functional enrichment from the upregulated genes in control and M4b mut melanophores/melanoma cells with respect to wildtype melanophores. (H) Dot plot depicting upregulated and downregulated processes in M4b mut melanoma with respect to control melanophores/melanoma *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 and ns P > 0.05.