ZFIN Image: Cai et al., 2025, Fig. 2

Image

Figure Caption

Fig. 2 The effect of PFOA on NIS mRNA levels and glycosylation of NIS protein in zebrafish larvae. A The zebrafish eggs were exposed to different PFOA concentrations for 7 days. Then, the mRNA levels were measured by real-time PCR. The treated groups were compared with the control group. n = 3. B zebrafish eggs were treated with different concentrations of PFOA for 7 days. NIS protein levels were analyzed using Western blot. GAPDH was used as a loading control for normalization. C The band intensities were calculated using Image J software. The ratios of glycosylated NIS to non-glycosylated NIS were plotted. one-way ANOVA with Tukey's HSD post hoc test，n = 3，**：p < 0.01, ***：p < 0.001; ****：p < 0.0001). The treated groups were compared with the control group.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Ecotoxicol. Environ. Saf.