ZFIN Image: Chamera et al., 2025, Figure 3

Image

Figure Caption

Figure 3

Characterization of the purified FL and TR Dr-tRNA-LC.A, binding curves of titration of purified tRNA-LC with fluorescently labeled zebrafish tRNA^Ile. Error bars indicate SD. n = 3 (independent dilution series, measurements of all three replicas are shown). B, size analysis by DLS of purified FL (green) and TR (blue) tRNA-LC in the presence or the absence of tRNA^Ile. n = 5 (technical replicates). C, first derivative of thermal melting curves obtained for purified FL (green; right) and TR (blue; left) tRNA-LC in the presence or the absence of tRNA^Ile. Inflection points used for T_m value calculation are indicated by vertical lines. n = 5 (technical replicates). D, quantification of the presented results. DLS, dynamic light scattering; Dr-tRNA-LC, ligase complex from Danio rerio; FL, full-length; rH, hydrodynamic radius; TR, truncated LC; tRNA-LC, tRNA ligase complex.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biol. Chem.