Fig. 3

Fig. 3

The locomotor activity of bcas3 KO zebrafish larvae. (A), The SPC frequence of 24 hpf zebrafish. SPC, spontaneous curls. (n = 10, per group). (B-D), The basic movement of zebrafish under light condition. (B), Representative image of trajectory and heat map. Quantitative analysis of distance (C) and average velocity (D) in 6 dpf WT and bcas3^−/− larvae. (n = 22, per group). (E), Graph illustrating the movement distance of 6 dpf zebrafish to alternating light-dark conditions, with periods consisting of 5-minute darkness and 5-minute light. The plot graph represents the movement distance of WT and bcas3^−/− larvae per minute. (n = 23, per group). (F), The average velocity of 6 dpf zebrafish during light-dark conditions with periods consisting of 55-minute darkness and 5-minute light. The plot graph represents the average velocity of WT and bcas3^−/− larvae per minute. (n = 23, per group). Data are shown as mean ± SEM. Unpaired Student’s t-test was used to analyze the SPC frequence, distance and average velocity. Significance levels are denoted as follows: ***P < 0.001