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Fig. 9 Gene mRNA and protein expressions of heme biosynthesis enzymes. (A) ALAS1, ALAD, HMBS, PPOX, and FECH gene expressions are suppressed in the female fch/fch liver. ALAS1, UROD, and FECH gene expressions are suppressed in the male fch/fch liver. (B) Standard immunoblotting was carried with similar protein loading for each of the displayed panels. The ALAS1, FECH, ALAD, and UROD protein expression is suppressed in the fch/fch livers. Under the experimental conditions used in this study, HMW aggregates of ALAD and UROD (and not ALAS1 or FECH) were detected in the fch/fch livers. There is an upward shift in ALAD and UROD monomers as indicated by the arrowheads. Data are shown as mean ± SD (n = 2–3 livers per group). Statistical analysis was performed by 1-way ANOVA test, ∗∗∗P < .001; ∗∗P < .01; ∗P < .05