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Fig. 5 Knockout of bmpr2a results in disruption of the circadian clock and behavioural rhythms, which are similar to those of amh mutants.The locomotor activities were monitored and analyzed in the bmpr2a−/− mutants and corresponding WT female zebrafish under LD (A–E) and DD (F–J) conditions. The total locomotor activities in 72 h (B, G), the quantification of average activities (C, H), and the moving distance and activity during the light-phase (D), dark-phase (E), subjective day (I) or subjective night (J) were shown. In the waveforms of locomotor activities, each point was calculated from the 5 min binned data across 3 days. For violin plots, dots and triangle correspond to individual zebrafish and the black and grey horizontal lines display the median as well as 25th and 75th percentiles. The box-and-whisker plots were presented as interquartile ranges with the median indicated by a line and whiskers extending from the minimum to the maximum values. For B–E n = 12 (WT) and n = 20 (bmpr2a−/−), G–J n = 12 (WT) and n = 24 (bmpr2a−/−). Statistical analysis was performed using two-sided unpaired t-test. K The quantification of the activity variance relative to respective WT among the amh−/−, bmpr2a−/−, or amh−/−;bmpr2a−/− mutants. L Cyclic expression of core clock genes in pituitaries harvested at the seven indicated time points across 24 h under DD condition from WT and bmpr2a−/− females. Target gene expression levels are shown relative to the beta-actin 2 reference gene actb2. The expression of WT CT22 was used for normalization. Data are plotted as mean values ± SD, n = 3, two-sided unpaired t-test. ZT represents zeitgeber time, where ZT 0 corresponds to lights on; CT represents circadian time. Source data are provided as a Source Data file.