FIGURE 4

FIGURE 4

Aβ promotes oligodendrocyte maturation via PKC, without changing total cell numbers. (A) Representative lateral images of the spinal cord of live transgenic larvae stably expressing olig2:EGFP and mbpa:TagRFPT, at 5 dpf. Dorsal and ventral regions are delineated by the dotted white line. (B) Graphs comparing the quantity of total olig2⁺ cells, (C) total mbp⁺ cells, (D) dorsal olig2⁺ cells, and (E) dorsal mbp⁺ cells. (F) Graphical representation of the progression of maturing mbp⁺ OLs (mbp⁺/olig2⁺ cells) throughout development in the total and (G) dorsal spinal cord. (H) Pie charts showing the percentages of dorsal mature OLs at 5 dpf. Scale bar = 20 μm. Data indicate means ± SEM, and dots represent individual larvae. *p < 0.05; Statistical significance was drawn by two‐way ANOVA and mixed‐effects analysis followed by Tukey's post hoc. n^{72 hpf} = 6–12 larvae per condition; n^{5 dpf} = 5–12 larvae per condition.