Reticulospinal cell labeling in four different transgenic lines. A, C, E, and G, MIPs from dorsal and sagittal views of an exemplary fish from A, vsx2 (n = 12), C, pcp4accu97Tg (n = 12), E, adcyap1bccu96Et (n = 11), and G, tiam2ay264Et (n = 12) at 6 dpf with RSNs labeled via retrograde dye injection. Scale bar is 100 μm. B, D, F, and H, Close ups at several planes to illustrate overlap between B, vsx2, D, pcp4accu97Tg, F, adcyap1bccu96Et, or H, tiam2ay264Et and RS backfill. Blue triangle in F, rostral panel indicates putative RoL1. Blue triangle in F and H, caudal panel indicates putative CaD and CaV cells. Scale bar is 25 μm.
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