Figure 4

Figure 4

Pyripyropene O targets the YY1/DR5 axis to induce apoptosis. (a) Molecular docking technique was used to reveal the specific binding mode of PyrO to DR5 protein and its intermolecular interactions. (b) Molecular docking technique was used to reveal the specific binding mode of PyrO to YY1 protein and its intermolecular interactions. (c,d) Effect of PyrO on the heat stability of YY1 protein. 10 μM PyrO treated PC-3 for 8 h. Cells were collected, and total protein was collected before analysing the heat stability of the protein using cellular thermal shift assay (CETSA). (e–g) Effect of PyrO on nuclear translocation of YY1. PC-3 cells were treated with PyrO for 20 h. Immunofluorescence staining was used to detect the localization of YY1 protein in the cells. ** p < 0.01 vs. Ctrl.