Fig. 6.

Fig. 6.

Impact of Tomm70^Ile525Thr mutation on C-start escape response dynamics. (A-C) The progression of movement traces and associated kinematic parameters for wt (A), heterozygous (B) and homozygous (C) mutant female zebrafish are illustrated during electrophysiological assessments. The top row presents sequential outlines of the zebrafish at 25 ms intervals, with the colour gradient corresponding to the adjacent colour bar. To emphasise the critical segment of the response, the 2 s window surrounding stimulus delivery (indicated by the grey dashed line on the colour bar) is rendered with increased opacity compared to the period 1-4 s after stimulus delivery. Scale bars: 10 mm. In the bottom row, large neural activity spikes are represented in a raster plot format, and the thrust magnitude and instantaneous spike frequency are depicted in the line graph beneath. The point of stimulus initiation is consistently marked by a grey dashed line for temporal orientation. (D-F) Box plots summarise spike latency (D), spike count (E) and median curvature amplitude (F). Black line denotes the median, the box denotes the interquartile range, whiskers extend to 1.5 times the interquartile range, and diamonds mark outliers. (D) Marked escalation in spike latency in heterozygous and homozygous female mutants relative to their wt female counterparts, with homozygous mutants displaying greater latency than heterozygous mutants. Analogous trends are noted in male mutants, with a notable reduction in spike latency for heterozygous males versus wt males. (E) Decrement in spike count for heterozygous and homozygous female mutants compared to that of female wt fish. (F) Reduction in median curvature amplitude for mutant females, with similar patterns observed in mutant males compared to that of their wt heterozygous counterparts. N=49 (female wt), 94 (female +/−) and 58 (female −/−), and N=42 (male wt), 56 (male +/−) and 74 (male −/−). Statistical assessments were performed using Fisher's permutation test. *P<0.05, **P<0.01, ***P<0.001.