ZFIN Image: Bochen et al., 2025, Fig. 2

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Figure Caption

Fig. 2

Transient receptor potential melastatin‐4 (TRPM4) knockout (KO) affects tumor spheroid formation, size and adhesion. (A) Representative microscopic images of tumor spheroids from DU145 cells and corresponding TRPM4 KO clones (n = 4). Scale bar indicates 100 μm. (B) Development of spheroid size over time was quantified by measuring the area of the core spheroid at the largest diameter (n = 4). (C) Statistical analysis of spheroid size on days 3, 5, and 10 postseeding. All individual values are plotted as dots in the graph. The median is shown as a red line, whiskers represent the interquartile range. Significance levels for data from both clones in C are given relative to DU145 cells. Differences between samples were analyzed using one‐way ANOVA followed by Dunnett's post‐hoc test.

Acknowledgments

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