Figure 2

Figure 2

Total distance traveled during 24 h, 14 h light and 10 h darkness, by zebrafish larvae. (A) The WT group exhibited a normal activity pattern, with more activity during the day than during the night. (B) asmt1^+/− experienced an advance in the activity phase, even at night. (C) asmt1^−/−, (D) asmt2^+/−, and (E) asmt2^−/− did not show an activity rhythm. (F) The total distance traveled decreased significantly in asmt2^−/− compared to the other groups. (G) asmt1^−/− and asmt2^+/− significantly decreased the distance traveled during the day compared to asmt1^+/− and asmt2^−/− compared to the rest of the groups. (H) Nighttime activity was significantly lower in asmt2^−/− than in the WT and the other mutant lines. Data are presented as mean ± SEM (n = 12 larvae/group). # p < 0.01 vs. asmt1^+/−; * p < 0.05 vs. asmt2^−/−; ** p < 0.01 vs. asmt2^−/−; *** p < 0.001 vs. asmt2^−/−. Unpaired t test.