Figure 10

Figure 10

(A,B) Representative confocal microscopy analysis of F-actin (Red) and integrin-β1 (green) distribution in MDA-MB-231 cells cultured under control conditions or treated with ZFEs for 96 h. Control cells exhibited a dense network of stress fibers (A). After ZFEs treatment, F-actin began to reorganize along the cell membrane, with lamellipodia and filopodia being nearly absent (B). Scale bars: 10 μm. (C,D) Representative confocal microscopy analysis of β-catenin (red) and E-cadherin (green) distribution in MDA-MB-231 cells cultured in control conditions or treated with ZFEs for 96 h. In untreated MDA-MB-231 cells, β-catenin was primarily localized in the cytosol and E-cadherin was completely absent (C). ZFEs treatment promoted the accumulation of β-catenin/E-cadherin complexes at the cell membrane, facilitating cell-to-cell contact and the reconstitution of an epithelial-like architecture (D). Scale bars: 20 μm.