Fig. 4 TFLS inhibits breast cancer cell metastasis in zebrafish and mouse models. (A) Zebrafish toxicity tests for determining the maximum safe concentration of TFLS. (B) Survival curves of zebrafish treated with TFLS. (C) Fluorescence intensity of MDA-MB-231 cells in zebrafish after six days of treatment. (D) Distribution of breast cancer cells in zebrafish following six days of TFLS intervention, with red fluorescence representing MDA-MB-231 cells. (E) Body weight curves of mice in TFLS-treated and model groups. (F) Bioluminescence imaging of 4T1−luc cells in mice at the end of the experiment. (G) Quantitative bar plot of fluorescence intensity of 4T1−luc cells in mice at the experiment’s conclusion. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01 compared to the model group.
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