Fig. 2

Fig. 2

Increased hyaloid vessel diameter in larvae of akr7a3^−/− eyes. (A–C) Representative confocal images and quantification of hyaloid vasculature in larvae at 5dpf. No significant difference was observed in branches among akr7a3^+/+ and akr7a3^−/− mutant larvae (B), however the diameter of hyaloid blood vessels was significantly increased in akr7a3^−/− mutants (C), white scale bar = 30 μm, n = 40/28, red arrows indicate branch points. (D–G) Representative confocal images and quantification of adult retinal vasculature. The vascular branches (E), sprouts (F), and vascular density (G) were not altered between adult akr7a3^+/+ and akr7a3^−/− zebrafish. Yellow arrows are branch points, red circles are sprouts. Red frame, high density area; Blue frame, low density area; Yellow frame, middle density area; Upper white scale bar = 350 μm, lower white scale bar = 100 μm, n = 7/8. Each data point represents vasculature in a 350 μm² area in the high-density region of the retina. Statistical analysis was performed by Student's t-test, The bars indicate mean ± SD values; dpf, days post fertilization.