Fig. 3

Fig. 3

Increased retinal vessel diameters and GBM thickening in adult akr7a3^−/− zebrafish. (A–D) Representative hematoxylin staining and quantification of retinal vasculature. The retinal blood vessel diameters of akr7a3^−/− eyes were significantly increased (B). The number of MC (C) and EC (D) was also increased. n = 40. Blue arrows, EC; green arrows, MC; Red arrows, RBC; Black scale bar = 100 μm. (E,F) Representative EM images and GBM quantification showed a significant increase in GBM thickness in adult akr7a3^−/− kidneys. n = 15/18. Black arrow, normal GBM of adult akr7a3^+/+ zebrafish, red arrow, thickened GBM of akr7a3^−/− zebrafish. Scale bar = 10 μm (left) and 2 μm (right), respectively. The bars indicate mean ± SD values. Statistical analysis was performed by Student's t-test. EC, endothelial cells; MC, mural cell; RBC, red blood cell; EM, electron microscopy; GBM, glomerular basement membrane.