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Fig. 3 Secondary assays analyzing enteric progenitor cell migration and intestinal transit in F0 crispants. (A, top) slc24a5 F0 crispant control and embryo schematic (A, bottom) show the bilateral streams of GFP+ enteric progenitor cells (EPCs, green) migrating posteriorly to populate the gut. The gray box outlines the region of the embryo (A, top) and scale bar denotes the distance measured between the EPC migration front and the end of the gut (migration progress). (B) Quantification of migration progress in F0 crispants for jarid2a, dlx1a, and mycn compared to F0 slc24a5 crispant controls showed no significant (ns) difference (two experiments, each data point equals one embryo). (C) Experimental set-up for the intestinal transit assay. Phenotypically sorted larvae are fed fluorescently labeled food at 6 and 7dpf. On day 7, 4?h post-feeding, larvae that have eaten are selected (?feeders?) and placed in a new dish without food. Sixteen hours later, clearance of labeled food from the gut is determined. In sox10 mutants (E) and jarid2a F0 crispants (F) fluorescently labeled food (green) is still visible in the guts compared to the cleared guts of controls (D). (G) Quantification of percent larvae with cleared guts (??2 experiments, % shown as mean?±?SEM, each data point equals one experiment, each experiment ??6 larvae). sox10 mutants and jarid2a F0 crispants have significantly lower percentages of larvae with cleared guts compared to controls. Asterisks show a significant difference to controls using an unpaired t-test (p?