ZFIN Image: Davidson et al., 2025, Fig. 1

Image

Figure Caption

Fig. 1 F0 crispants targeting ret, sox10, or phox2bb recapitulate known ENS phenotypes. (A?E) F0 crispants targeting ret phenocopy the lack of phox2bb:GFP+ ENS cells (green) in stable ret mutant larvae (C) in comparison to wildtype-level ENS cells in control larvae (A). Compare ENS phenotypes between (C) and (D), arrows point to remaining ENS neurons in the anterior gut. (E) A small portion of ret F0 crispants show ENS cell reduction (B) Schematic of zebrafish larvae, the gray boxed area indicates the area shown in A-M. Line indicates length used for quantification of anterior neuron numbers in ret crispants in (F). (F) Significantly fewer GFP+ cells are present within 200 um of anterior gut in ret F0 crispants compared to control injected larvae (p?

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neurogastroenterol. Motil.