Image
|
Figure Caption
Fig 6
Non-Denaturing gel demonstrating alignment and extension of ssDNA. zPol θ PD (20 nM) was preincubated with 30 nM 5’-FAM 12-mer ssDNA in reaction buffer. Samples either had all nucleotides (+dNTP) or no nucleotides (-dNTP) added and the ternary complex was incubated for 45 minutes at 37°C. Reactions were stopped and products separated on a 12% Native PAGE. The gel was visualized on a Typhoon scanner. MMEJ products are indicated, as well as smaller snap-back products indicated by the bracket.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ PLoS One