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Fig. 3 creb3l1TA+/TA+ zebrafish exhibit defects in caudal fin ray bifurcation. a Fins of 6 mpf creb3l1+/+Tg(Sp7:eGFP/+) and creb3l1TA+/TA+Tg(Sp7:eGFP/+) fish were examined by direct fluorescence. The length of fins was measured from the caudal peduncle to the tip of the 3rd ray in both lobes (white lines). Bifurcation depth of the 2nd and 3rd ray was measured from the caudal peduncle to the bifurcation point in both lobes (pink lines) and averaged. Primary bifurcations are marked with white arrows. Orange line demarcates the region used to quantitate Sp7+ area (all area distal to the orange lane). Scale bar = 1 mm. b Higher magnification of representative images of caudal fins from creb3l1+/+Tg(Sp7:eGFP/+) and creb3l1TA+/TA+Tg(Sp7:eGFP/+) fish. Red arrows point to secondary bifurcations of 3rd sister ray, while blue arrows points to a secondary bifurcation of 5th sister ray in wild-type, but not in mutant tail. c The lengths of tails are not significantly different between creb3l1+/+Tg(Sp7:eGFP/+) and creb3l1TA+/TA+Tg(Sp7:eGFP/+ fish when analyzed by an unpaired t-test with Welch’s correction. N = 14–15. Each data point represents an individual fish. d The Sp7+ area/tail is not significantly different between creb3l1+/+Tg(Sp7:eGFP/+) and creb3l1TA+/TA+Tg(Sp7:eGFP/+) fish when assessed by an unpaired t-test. N = 13–23. Each data point represents an individual fish. e The distance from caudal peduncle to the first point of bifurcation is significantly increased in creb3l1TA+/TA+Tg(Sp7:eGFP/+) fins relative to creb3l1+/+Tg(Sp7:eGFP/+) fish when analyzed by an unpaired t-test. ***p ≤ 0.001. N = 13–16. Each data point represents an individual fish. f The total number of bifurcations per fin were assessed and show that creb3l1TA+/TA+Tg(Sp7:eGFP/+) fins have significantly fewer bifurcations/fin relative to creb3l1+/+Tg(Sp7:eGFP/+) fins when assessed by non-parametric Mann-Whitney test. **p ≤ 0.01. N = 13–16. Each data point represents an individual fish.