ZFIN Image: Thomas et al., 2025, Fig 7

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Figure Caption

Fig 7 zPol θ PD is able to bypass DNA lesions.

Denaturing gel demonstrating primer extension on damaged dsDNA substrates with MgCl₂ and MnCl_2.As described in the Materials and Methods, 200 nM hPol θ and zPol θ PDs were preincubated with 50 nM DNA substrate, (A) Undamaged (TT), (B) CPD (TT), (C) Abasic ((AP)T), (D) 8-oxo-dG ((8-oxo-dGT)T). Reactions were initiated by the addition of 125 nM dNTPs as described and either 10 mM MgCl₂ or MnCl₂. Reactions were carried out at 37°C for 5 minutes and products visualized on a 12% denaturing gel. Higher migrating products are indicative of full extension (n+12) with smeared bands representing de novo synthesis with extension past n+12.

Acknowledgments

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