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Fig. 6 Representative images of developing zebrafish embryos at 9 hpf after exposure to 0 or 50 μg/L AS1842856 (A). Abnormal epiboly in embryos was indicated by black arrow. Morphological characteristics of normal (control) and abnormal (50 μg/L AS1842856) epibolic embryos at different time points from 9 to 72 hpf (B). Mortality of zebrafish embryos at 24 hpf (C) and 72 hpf (D), malformation rate of zebrafish embryos at 9 hpf (E), 24 hpf (F), and 72 hpf (G) after exposure to 0 or 50 μg/L AS1842856, n = 3. Expression of ripor2 in 9 hpf embryos following exposure to 0 or 50 μg/L AS1842856 (H, n = 6, two technical replicates for each experimental replicate). *p < 0.05 was considered to have a statistically significant difference.