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Fig. 4 Representative images of developing zebrafish embryos at 9 hpf following microinjection with empty vector or shripor2 (A). Abnormal epiboly in embryos was indicated by a black arrow. Morphological characteristics of normal (empty vector injection) and abnormal (shripor2 injection) epibolic embryos at different time points from 9 to 72 hpf (B). Mortality of injected zebrafish embryos at 24 hpf (C) and 72 hpf (D). Malformation rates of injected zebrafish embryos at 9 hpf (E), 24 hpf (F), and 72 hpf (G). Expression of ripor2 in 9 hpf embryos following injection with an empty vector or shripor2 (H), n = 5. *p < 0.05 was considered to have a statistically significant difference.