ZFIN Image: Garcia-Peiro et al., 2025, Fig. 6

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Figure Caption

Fig. 6 a) Schematic timeline of the Pt(0)-mediated prodrug activation in vivo assay (created with BioRender). dpf: days post-fertilization; hpi: hours post-injection. b) Confocal microscopy images of nuclei-labeled HCT116 cells (cyan) implanted in the PVS of 2-dpf zebrafish larvae after 2 days of incubation in E3 medium with or without pro-SN38. Groups: non-Pt-treated cells+pro-SN38 (left); PEG-dPt-2-treated cells+DMSO (middle), and PEG-dPt-2-treated cells+pro-SN38 (left). N=9–12. The dashed line represents the tumor area. Scale bars=50 μm. BgF: Background fluorescence. c, Measurement of tumor size between groups after treatment. d) Analysis of the number of nuclei-labeled cancer cells between groups after treatment. e) Quantitative analysis of green fluorescence signal (Ex=514 nm). Statistical analysis: one-way ANOVA followed by Tukey's post-hoc test: ns>0.05, * P≤0.05, ** P≤0.01, *** P≤0.001.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Angew. Chem. Int. Ed. Engl.