Figure 3

Figure 3

The effects of helenalin, (helenalinyl)malonate (BHM), and bis(helenalinyl)glutarate (BHG) on cell proliferation of MDA-MB-231 through MYC-dependent pathway. (a) MYC expression under helenalin, BHM, and BHG 72 h treatments in MDA-MB-231 cells was determined by enzyme-linked immunosorbent assay (ELISA). (b) Relative MYC levels in MDA-MB-231 cells transfected with scrambled siRNA (negative control) or MYC siRNA by western blotting. The uncropped blots are shown in the Supplemental Materials. (c) The cytotoxic effect of helenalin, BHM, and BHG in MDA-MB-231 cells with or without MYC siRNA transfection. Cytotoxicity was detected after a 72-h treatment. JQ1 was used as a positive control of MYC inhibitor. Statistical differences were evaluated by Student’s t-test. * denoted p < 0.05 as compared to untreated control in (b) and as compared to MDA-MB-231 cell line in (c). Data presented as mean ± standard error of at least two experiments, each in duplicate.