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Fig. 3 a?d, Genome coverage plots show differential accessibility of Hs_GAS8-p1 and Hs_NRP2-p1 in human outer-ear versus nose cartilage (a) and differential accessibility of Hs_BMP2-p1 in human nose versus outer-ear cartilage (c). Numbers along x axes (a,c) indicate genomic coordinates. Chr., chromosome. Hs_GAS8-p1:GFP and Hs_NRP2-p1:GFP show restricted transgenic activity in zebrafish gill filaments at 14 d.p.f. (b). Hs_BMP2-p1:GFP shows restricted activity in zebrafish hyaline cartilage (d). e, Dr_ucmaa-p1:GFP shows restricted transgenic activity in zebrafish gill-filament cartilage; sox10:dsRed labels all cartilage in the merged images. f, Whole-mount X-gal staining shows that Dr_ucmaa-p1:lacZ drives reporter activity in the mouse outer ear (black arrow) at E15.5. g, Horizontal section through the outer ear shown in f, counterstained with nuclear fast red, shows expression in the pre-cartilage condensations (arrow) of the outer-ear canal but not in middle-ear hyaline cartilage (arrowhead). Scale bars, 50?µm.