Fig 6

Fig 6

Identification of the VPA1365-binding motifs. (A) EMSA analysis of VPA1365 binding to the scrG promoter with different truncations. Six truncated fragments for the scrG promoter (left panel) were analyzed for their interaction with VPA1365 (right panel). (B) Nucleotide sequences (271 bp) of the scrG promoter (PscrG). The VPA1365-binding site is boxed in black, while −35 box and −10 box sequences are underlined. Start codon (ATG) and +1 of transcription are shown in red and with an arrow, respectively. (C) EMSA analysis of VPA1365 binding to the truncated ∆scrG promoter (∆PscrG) with the deletion of the VPA1365-binding site (140–166 bp). The fragment of ∆PscrG (left panel) was analyzed for its binding with VPA1365 (right panel). (D) The predicted consensus VPA1365-binding motif was generated from MEME-Suite analysis of the selected promoters.