ZFIN Image: Yin et al., 2025, Fig 3

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Fig 3

The effects of vpa1365 on the expression and secretion of T3SS2. (A) Relative transcript levels of the T3SS2-associated genes in WT, ∆vpa1365, ∆vpa1365-vpa1365, ∆vpa1365-vtrA, and ∆vpa1365-vtrB. Results are represented as mean ± SD (n = 3, biologically independent experiments). *P ≤ 0.05 and **P ≤ 0.01. ns, no significance. (B) The T3SS2 translocon protein VopD2 secretion and RNAP of WT, ∆vpa1365, and complemented strains in LB medium with or without 0.04% bile salts. (C) The analysis of binding of VPA1365 to the promoter of the regulator vtrA. (D) The T3SS2 translocon protein VopD2 secretion and RNAP of WT, ∆vpa1365, and complemented strains in LB medium at 28°C, 40°C, pH = 8, 0.1 M NaCl, and 0.5 M NaCl, respectively.

Acknowledgments

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