Figure 6

Figure 6

The intracellular calcium agonist AdA has a synergistic effect with LIPUS in regulating inflammatory chondrocyte. Calcium transient relative fluorescence intensity of ROIs in LIPUS alone A), in AdA alone B), in the combination of LIPUS and AdA C). Quantification of the number D) (n = 8), the magnitude E) (n = 8) of calcium peaks, and the responsive rate of cells F) (n = 4). WB G) and densitometry analysis of LC3 H) protein expression of chondrocyte co‐incubated with AdA following LIPUS treatment (n = 3). WB I) and densitometry analysis of Collagen II J) and MMP‐13 K) protein expression of chondrocyte co‐incubated with AdA following LIPUS treatment (n = 3). L) Immunofluorescence was used to detect the expression of Collagen II and MMP‐13 after the chondrocytes was co‐incubated with AdA. Scale bar, 20µm. Quantification the relative fluorescent intensity of Collagen II M) and MMP‐13 N) in chondrocytes (n = 3). O) Schematic diagram of LIPUS alleviates OA via calcium signaling‐dependent autophagy of chondrocyte. Data are presented as means ± SD. Statistical analysis was performed using Student's t test. ****(P < 0.0001), ***(P < 0.001), **(P < 0.01), *(P < 0.05), ns (0.05 < P).