Figure 2

Figure 2

LIPUS promotes chondrocyte calcium oscillation of living zebrafish. A) Alcian blue staining to label the cranial cartilage structure of zebrafish juveniles. B) Zebrafish larvae were immobilized in a confocal dish with low‐melting‐point agarose. C) Schematic of the real‐time LIPUS‐processed calcium imaging under a confocal microscope. D) The whole fish, cranial and ceratohyal observed under a confocal microscope. E) 5 chondrocytes were circled with different colors as ROIs. F) The real‐time fluorescence intensity of the five ROIs. G) Calcium transient relative fluorescence intensity of ROIs. Quantification of the number H) (n = 5), the magnitude I) (n = 5) of calcium peaks. Data are presented as means ± SD. Statistical analysis was performed using Student's t test. **(P < 0.01), *(P < 0.05), ns (0.05 < P).