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Fig. 4 F0 emergence rank as a predictor for larval behaviour. Behavioural parameters obtained during a Light Dark Challenge (LDC) test, in larval (5dpf) fish of the F1 (A–H) and F2 (I, J) generation during the baseline phase (A–D), the dark phases (pooled; E–H) and light and dark phases (pooled; I, J), plotted over the emergence ranks of the F0 parental fish during a group emergence test: swimming velocity (V; 4A, y = − 0.08x + 3.65, r2 = 0.06, 3E, y = − 0.07x + 4.52, r2 = 0.05), acceleration (4B, y = − 0.004x + 0.11, r2 = 0.04, 4F, y = − 0.004x + 0.14, r2 = 0.02), angular velocity (Ω; 3C, y = 1.05x + 69.29, r2 = 0.05, 3G, y = 1.39x + 19.60, r2 = 0.04, 3I, Y = 1.081*X + 57.13, r2 = 0.05), and state of immobility (MSi; 3D, y = 0.13x + 21.25, r2 = 0.08, 3H, y = 0.04y + 9.92, r2 = 0.08, 3 J, Y = 0.02x + 10.01, r2 = 0.02). These parameters yielded a significant correlation with the F0 emergence ranks [Spearman rank test, N = after (before outlier elimination)]