Figure 6

Figure 6 Dopamine acts through intracellular secondary messenger cAMP in neuroepithelial cells (NECs)

A, addition of SQ22536, an adenylyl cyclase (AC) inhibitor, decreased the effect of hypoxia on intracellular Ca²⁺. B, summary data treated as in (A) showing the mean ± SD F/F₀ during the first 2 min of hypoxia exposure (Hypoxia‐1), the reduction in the mean ± SD F/F₀ during the entire duration of SQ22536 exposure (Hypoxia‐2 + SQ22536; Kruskal–Wallis test, P = 0.010, n = 6 cells) and the mean ± SD F/F₀ during the last 2 min of hypoxia exposure (Hypoxia‐3). C, forskolin, an AC activator, partially recovered the suppressive effect of dopamine on the Ca²⁺ response to hypoxia. D, summary data treated as in (C) showing recovery in the mean ± SD F/F₀ of the hypoxic response from dopamine with forskolin (Kruskal–Wallis test, P = 0.002, n = 6 cells). The response to hypoxia fully recovered following both treatments (Kruskal–Wallis test, P > 0.999, n = 6 cells). [Colour figure can be viewed at wileyonlinelibrary.com]