Figure 3

Figure 3 Extracellular Ca²⁺ contributes to the response to hypoxia in neuroepithelial cells (NECs)

A, Ca²⁺ imaging trace from a GCaMP‐containing NEC, where the response to hypoxia was reduced with the addition of 100 µm nifedipine, an L‐type Ca²⁺ channel blocker. The response to hypoxia was fully recovered after a 15 min washout (break in trace). B, Summary data from NECs treated as in (A) illustrating a reduction in the mean ± SD F/F₀ (Kruskal–Wallis test, P = 0.006, n = 6 cells). Three cells were evaluated for recovery after a 15 min washout period (Kruskal–Wallis test, P > 0.999, n = 3 cells). C, Ca²⁺ imaging trace from a GCaMP‐containing NEC, where the response to hypoxia was reversibly reduced when Ca²⁺ was removed from the extracellular solution. D, summary data from NECs treated as in (C) showing a reduction in the mean ± SD F/F₀ (Kruskal–Wallis test, P < 0.006, n = 6 cells). The response to hypoxia fully recovered after zero Ca²⁺ treatment (Kruskal–Wallis test, P > 0.999). [Colour figure can be viewed at wileyonlinelibrary.com]