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Fig. 5 Selenon knock down cells present increased ROS levels when compared to control. (A) Flow cytometry assay of CM-H2DCFDA stained cells to measure ROS levels in selenon knock down (KD1 and KD8) and WT control cells. (B) Quantification of fluorescence from (A) reveals significant increase of ROS levels in selenon KD cell lines when compared to control. (C) Immunoblotting of protein carbonylation and (D) nytrosylation demonstrate increased levels in selenon KD when compared to control cells
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