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Fig. 5 FRAP simulations with varying image connectivity. (A) OT images with altered dead-end percentage. (B) Results from FRAP experiments by simulating the particle model on OT images with dead ends of varying volume fraction α and dead ends. (C) with altering volume fraction, α, for different environments and varying connectivity. The green box highlights that adding dead ends reduced the diffusion coefficient more drastically than reducing volume fraction of connected spaces. Here, the altered OT images with dead ends were dilated across the entire stack so that α was kept relatively similar to the connected OT image.