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Fig. Ext Data 3 a Selected maximum intensity projections of transmission images of the larval zebrafish tail over 11 hours of observation, starting at 2.5 days post-fertilization. The blue dashed line indicates the tip of the vasculature at the end of the tail, growing around 100 μm over the 11 hours window of observation (blue arrows). b Despite this growth, self-driving microscopy kept the vascular region of interest in focus over the observation window. Maximum intensity projections of the corresponding high-resolution region showcase details of the outgrowth of a single vessel (white arrowhead) with subsequent anastomosis (white arrows) with a neighboring vessel, including positioning of endothelial nuclei (asterisk). The vasculature was labeled with Tg(kdrl:EGFP). c Maximum intensity projection of the entire field of view of the low-resolution acquisition at start of the time-lapse imaging, with the inset highlighting the region used for tracking. d Representative X–Y, Y–Z, X–Z maximum intensity projections used in the region tracking algorithm. Scale bar lengths are as follows: a, c, d 150 μm; b 50 μm.